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Peptide Reconstitution Calculator

Calculate the exact concentration, syringe volume, and units to draw after reconstituting your peptide vial with bacteriostatic water.

Used by 10,000+ researchers
Enter Your Peptide Details
Your Results
Concentration
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Volume to Draw
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Syringe Units to Draw
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Doses Per Vial
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Step by Step

How to Use the Peptide Calculator

1

Select Your Peptide

Choose from our pre-loaded library of common research peptides. The calculator will auto-fill the standard vial size. Select "Custom" to enter your own values.

2

Enter Bacteriostatic Water Volume

Enter the amount of bacteriostatic water (in mL) you plan to add to the vial. Common amounts are 1 mL or 2 mL. More water means a less concentrated solution and larger injection volume per dose.

3

Set Your Desired Dose

Enter the dose you want per injection in micrograms (mcg). The calculator will pre-fill a common research dose for the selected peptide, but you can adjust it to match your protocol.

4

Read Your Results

The calculator instantly shows your concentration (mcg/mL), the volume to draw in mL, the number of units on your insulin syringe, and how many total doses you'll get from the vial.

Essential Knowledge

Understanding Peptide Reconstitution

Reconstitution is the process of mixing a freeze-dried (lyophilized) peptide powder with bacteriostatic water to create an injectable solution. Getting this step right is critical for accurate dosing.

💧 Why Bacteriostatic Water?

Bacteriostatic water contains 0.9% benzyl alcohol, which prevents bacterial growth. This allows you to use the reconstituted vial multiple times over several weeks, unlike sterile water which is single-use only.

🌡️ Storage After Mixing

Once reconstituted, store your peptide vial in the refrigerator at 2-8°C (36-46°F). Most peptides remain stable for 3-4 weeks. Never freeze a reconstituted vial and keep it away from direct light.

💉 Injection Technique

When adding bacteriostatic water, aim the stream against the glass wall of the vial — never directly onto the peptide cake. Let it dissolve slowly. Gently swirl if needed, but never shake.

📏 Syringe Selection

For most peptide doses, a 100-unit (1 mL) insulin syringe works well. For smaller, more precise doses, a 50-unit (0.5 mL) or 30-unit (0.3 mL) syringe gives better accuracy with finer graduations.

Common Questions

Peptide Reconstitution FAQ

Draw your desired amount of bacteriostatic water into a syringe, then inject it slowly into the peptide vial by aiming the stream against the glass wall. Let it dissolve gently — never shake the vial. Swirl lightly if needed. Once fully dissolved, your peptide is ready to dose.
The most common amounts are 1 mL or 2 mL of bacteriostatic water per vial. Using less water gives a more concentrated solution (fewer units per dose), while more water makes dosing easier with larger syringe volumes. Use the calculator above to see how your water volume affects your dose.
A 100-unit (1 mL) insulin syringe holds 1 mL total, with each tick mark representing 1 unit (0.01 mL). A 50-unit (0.5 mL) syringe holds half that, but each tick mark still represents 1 unit (0.01 mL) with finer graduations for more precision. A 30-unit (0.3 mL) syringe offers the most precision for very small doses. Choose based on your typical dose volume.
Divide the total peptide in the vial (in mcg) by the amount of bacteriostatic water added (in mL). For example, a 5 mg vial (5,000 mcg) reconstituted with 2 mL of water gives a concentration of 2,500 mcg per mL. This means each 0.1 mL (10 units on a 100-unit syringe) contains 250 mcg.
Divide the total peptide content (in mcg) by your per-dose amount (in mcg). For example, a 5 mg BPC-157 vial (5,000 mcg) dosed at 250 mcg per injection gives you 20 doses per vial. The calculator above shows this automatically based on your inputs.
BPC-157 (Body Protection Compound-157) is a synthetic peptide derived from a protein found in gastric juice. It is commonly researched for tissue repair and recovery support. Typical research doses range from 200 to 500 mcg per injection, administered subcutaneously once or twice daily. A standard vial comes in 5 mg.
Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which allows the reconstituted peptide to remain sterile for multiple uses over days or weeks. Sterile water has no preservative and should only be used for single-use applications. For multi-dose vials, bacteriostatic water is strongly recommended.
Store reconstituted peptides in the refrigerator at 2-8°C (36-46°F). Keep the vial upright and avoid freezing. Most reconstituted peptides remain stable for 3-4 weeks when stored properly with bacteriostatic water. Unreconstituted (lyophilized) peptides can be stored frozen for longer shelf life.